Design probes and oligos
with thermodynamics you can trust

Modification-aware Tm prediction for diagnostic probes and oligonucleotide therapeutics

The precision standard for molecular assay design

PCR · qPCR multiplex panels · SNP genotyping · LNA probes · Isothermal amplification · NGS hybrid capture

Multiplex balancing

AccuMatch™ harmonizes melting profiles across dozens of primers and probes so every reaction fires evenly.

Mismatch discrimination

We predict binding free energy (ΔG) to discriminate single-nucleotide differences to 1.0 °C.

Buffer-aware design

AccuMatch™ tunes predictions to your actual buffer — so in-silico designs work on the first plate.

The precision standard for oligonucleotide therapeutic design

ASOs · siRNAs · sgRNAs · LNA · 2′-O-Me · phosphorothioate · AEGIS

Modified-chemistry precision

AccuMatch™ extends nearest-neighbor thermodynamics to modified bases — so duplex stability predictions hold up at the bench.

Faster lead optimization

Triage millions of candidate sequences in silico and send only the top binders to synthesis.

Off-target screening

AccuMatch™ flags binding mismatches early, so safety liabilities are caught before lead selection — not after a failed tox study.

How it works

AccuMatch™ predicts the melting temperature of a probe against its target sequence using nearest-neighbor thermodynamics — the single physical property that determines whether your probe will bind, miss, or misfire.

Every 0.1 °C matters

Every 0.1 °C matters. ΔG dictates whether a probe binds, drops out, or cross-reacts. Even a 1 °C miss can mean dead reactions or off-target hits. AccuMatch™ predicts duplex stability with sub-degree accuracy — small numbers, large consequences.

AccuMatch™ differentiator

A 20-year jump in accuracy. The standard nearest-neighbor parameters most tools rely on come from a 1998 paper with mean error around 0.5 °C. AccuMatch™ delivers up to 7 °C improvement on modified substrates — the first meaningful advance in over two decades.