Design probes and oligos
with thermodynamics you can trust.

Modification-aware Tm prediction for diagnostic probes and oligonucleotide therapeutics.

The precision standard for molecular assay design

PCR · qPCR multiplex panels · SNP genotyping · LNA probes · Isothermal amplification · NGS hybrid capture

Multiplex balancing

Primer-dimers and uneven Tm cause cross-reactivity and dropouts in high-plex panels. AccuMatch™ harmonizes melting profiles across dozens of primers and probes so every reaction fires evenly.

Mismatch discrimination

Clinical assays can’t afford false positives from near-match binding. We predict binding free energy (ΔG) to discriminate single-nucleotide differences to 1.0 °C.

Buffer-aware design

Salt, cosolvents, and master mixes shift binding physics away from textbook values. AccuMatch™ tunes predictions to your actual buffer — so in-silico designs work on the first plate.

The precision standard for oligonucleotide therapeutic design

ASOs · siRNAs · sgRNAs · LNA · 2′-O-Me · phosphorothioate · AEGIS

Modified-chemistry precision

Standard predictors break on LNA, 2′-O-Me, and phosphorothioate backbones. AccuMatch™ extends nearest-neighbor thermodynamics to modified bases — so duplex stability predictions hold up at the bench.

Faster lead optimization

Trial-and-error wet-lab screening burns months and capital. Triage millions of candidate sequences in silico and send only the top binders to synthesis.

Off-target screening

Cross-hybridization risk surfaces late in preclinical and kills programs. AccuMatch™ flags binding mismatches early, so safety liabilities are caught before lead selection — not after a failed tox study.

How It Works

Our platform enables scientists to model gene probe sequences, and through our statistical algorithms, to predict the melting temperature of the gene probe, to the gene. Melt temperature is the property most critical to gene probe success.

Every 0.1°C Matters

Because ΔG dictates efficacy, precision down to every 0.1°C makes a difference. When probe strength is not matched to the gene, efficiency, specificity, and performance degrade quickly. Our algorithms provide hyper-accurate fractional thermodynamic calculations that prevent off-target binding.

AccuMatch™ Differentiator

DNA Analytics is the first to improve match strength algorithms in over 20 years. Our best-case improvements are up to 7.0° C, compared to the 1998 landmark study of 0.5° C.