Design probes and oligos
with thermodynamics you can trust.

Modification-aware Tm prediction for diagnostic probes and oligonucleotide therapeutics.

The precision standard for molecular assay design

PCR · qPCR multiplex panels · SNP genotyping · LNA probes · Isothermal amplification · NGS hybrid capture

Multiplex balancing

Primer-dimers and uneven Tm cause cross-reactivity and dropouts in high-plex panels. AccuMatch™ harmonizes melting profiles across dozens of primers and probes so every reaction fires evenly.

Mismatch discrimination

Clinical assays can’t afford false positives from near-match binding. We predict binding free energy (ΔG) to discriminate single-nucleotide differences to 1.0 °C.

Buffer-aware design

Salt, cosolvents, and master mixes shift binding physics away from textbook values. AccuMatch™ tunes predictions to your actual buffer — so in-silico designs work on the first plate.

The precision standard for oligonucleotide therapeutic design

ASOs · siRNAs · sgRNAs · LNA · 2′-O-Me · phosphorothioate · AEGIS

Modified-chemistry precision

Standard predictors break on LNA, 2′-O-Me, and phosphorothioate backbones. AccuMatch™ extends nearest-neighbor thermodynamics to modified bases — so duplex stability predictions hold up at the bench.

Faster lead optimization

Trial-and-error wet-lab screening burns months and capital. Triage millions of candidate sequences in silico and send only the top binders to synthesis.

Off-target screening

Cross-hybridization risk surfaces late in preclinical and kills programs. AccuMatch™ flags binding mismatches early, so safety liabilities are caught before lead selection — not after a failed tox study.

How It Works

Our platform enables scientists to model gene probe sequences, and through our statistical algorithms, to predict the melting temperature of the gene probe, to the gene. Melt temperature is the property most critical to gene probe success.

Every 0.1°C Matters

Because ΔG dictates efficacy, precision down to every 0.1°C makes a difference. When probe strength is not matched to the gene, efficiency, specificity, and performance degrade quickly. Our algorithms provide hyper-accurate fractional thermodynamic calculations that prevent off-target binding.

AccuMatch™ Differentiator

DNA Analytics is the first to improve match strength algorithms in over 20 years. Our best-case improvements are up to 7.0° C, compared to the 1998 landmark study of 0.5° C.

How We Work

Keep It Confidential

We get it, confidentiality is imperative! Before anyone shares anything. we’ll complete a non-disclosure agreement (NDA) to ensure your protection.

The Right Fit

Every need is unique. We’ll work with you to understand your goals and capabilities to identify the right-fit solution, whether it’s the patented AccuMatch Engine Cloud/SaaS solution or the creation of a new Assay Design Pipeline or System.

AccuMatch In Action

See our AccuMatch product demo or setup a custom demo on your own dataset for a live comparison of DNA Analytics’ outputs versus your existing system pipeline.

Delivery And Support

With AccuMatch deployed in the AWS Cloud, our software is delivered quickly. For custom solutions, our design and engineering team will support you through the entire process.

Meet The Team Behind

Our Patent-pending Algorithms

Contact Us

Let's Talk

If you would like to talk with us, please click here to make an appointment.

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